Associate Provost for Research Boston University Medical Campus
NIDCR - National Institute of Dental and Craniofacial Research	Research Resources

Abstract

Abstract: The Biological Core will be responsible for extracting and purifying fimbriae, LPS and lipid A from Porphyromonas gingivalis. Standard biochemical techniques will be employed for these procedures. The core will also construct the P. gingivalis fimA deletion mutant. In addition, the core will coordinate the commercial production of polyclonal and monoclonal antibodies to P. gingivalis fimbriae, to the endothelial receptor, to Toll-like proteins and moesin. Finally, the biological core will coordinate sample preparation for nucleotide and peptide sequencing and flow cytometry.

Thesaurus Terms:
Bacteroides gingivalis, biomedical facility, lipopolysaccharide
flow cytometry, immunologic assay /test, monoclonal antibody, nucleic acid sequence, pilus, protein sequence

Institution:	BOSTON MEDICAL CENTER
	ONE BOSTON MEDICAL CENTER PLACE
	BOSTON, MA 02118
Fiscal Year:	2004
Department:
Project Start:
Project End:
ICD:	NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
IRG:	ZDE1